LH Ovulation Rapid Test Midstream
INTENDED USE
The LH Ovulation Rapid Test Midstream (Urine) is a rapid chromatographic immunoassay for the qualitative detection of luteinizing hormone (LH) in urine to aid in the detection of ovulation.

SUMMARY
Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. In order for pregnancy to occur, the egg must be fertilized by sperm within 24 hours after its release. Immediately prior to ovulation, the body produces a large amount of luteinizing hormone (LH) which triggers the release of a ripened egg from the ovary. This “LH surge” usually takes place in the middle of the menstrual cycle. The LH Ovulation Rapid Test Midstream (Urine) is a complete system to help predict the time of ovulation, and peak fertility. It is during this fertile time that pregnancy is most likely to occur. The LH Ovulation Rapid Test Midstream (Urine) detects the LH surge in urine, signaling that ovulation is likely to occur in the nest 24-36 hours. The test utilizes a combination of antibodies including a monoclonal LH antibody to selectively detect elevated levels of LH. Important: The LH surge and ovulation may not occur in all menstrual cycles.

PRINCIPLE
Ovulation is the release of an egg from the ovary. The egg then passes into the fallopian tube where it is ready to be fertilized. In order for pregnancy to occur, the egg must be fertilized by sperm within 24 hours after its release. Immediately prior to ovulation, the body produces a large amount of luteinizing hormone (LH) which triggers the release of a ripened egg from the ovary. This is known as an “ LH surge”, and usually takes place in the middle of the menstrual cycle. It is during this fertile time that pregnancy is most likely to occur. It is important to note that an LH surge and ovulation may not occur in all cycles.

The LH Ovulation Test is a rapid lateral flow immunoassay for the qualitative detection of LH surges in urine, signaling that ovulation is likely to occur in the next 24-36 hours.

The test utilizes a combination of antibodies including a monoclonal LH antibody to selectively detect elevated levels of LH.

SPECIMEN COLLECTION AND PREPARATION
Determine the optimal time for urine collection. For best results, collect urine at about the same time each day. Some women have found that their best specimen is after 12 noon. Do not collect the first urine specimen after waking up
Reduce liquid intake approximately 2 hours prior to urine collection.
Urine can be stored at room temperature for up to 8 hours or at 2-8˚ C for up to 24 hours. Do not freeze. For best results, test urine on the same day that it is collected. If refrigerated, let urine reach room temperature before testing. Do not shake the container. If a sediment forms at the bottom of the collection container, allow the sediment to settle. Use only urine from the top of the container.
PRECAUTIONS
Do not use after the expiration date printed on the foil pouch
Do not use if pouch is torn or damaged
Store in a dry place at 4-30˚ C OR 39.2 – 86 ˚F. Do not freeze.
Keep out of the reach of children.
Do not open the test foil pouch until you are ready to start the test
For in vitro diagnostic use. Not to be taken internally.
The used test should be discarded according to local regulations
INSTRUCTIONS
WHEN TO START TESTING
This kit contains 5 tests, allowing you to test over several days to find your most fertile time. Calculate when to start testing using the chart below.

1) First, determine your Menstrual Cycle Length. Your Menstrual Cycle Length is the number of days from the first days of your period (menstrual bleeding) to the last day before the nest period starts.
2) Next, determine theDays to Count Ahead after the period to start testing. Find the menstrual cycle length on the first row of the chart below, and read the corresponding number in the second row. This is the number of days after your period to begin testing.
3) See the Example and Sample Calendar below to determine which day you should begin testing. First morning urine shouldNOT be used when testing for LH. For best results, you should test around the same time each day. You should reduce your liquid intake approximately 2 hours prior testing.
Example: My usual cycle length is 28 days. My last period started on the 3rd. The “When to start Test Chart” shows that I will count ahead 11 days beginning with the 3rd. When I count 11 days ahead on the calendar, I find that I will collect and test my urine starting on the 13th.

First day of the last period
Beginning testing with the LH Ovulation Test